Nucleic Acid Sequencing Platform
Milton Yutaka Nishiyama Jr., Bioinformatician
Mariana Salgado Morone
The Biodiversity Genomics Unit (UGBio) performs researches on genomics and transcriptomics focusing mainly on the study on animals that produce venoms and biologically active molecules. Nucleic acid sequencing techniques, from those develop by Gilbert and Sanger in 1977 to the technologies called NGS (Next Generation Sequencing), are applied for this goal.
After the implantation of CeTICS, supported by FAPESP, a facility lab was established enclosing adequate equipment for this kind of analysis. A technician and researcher team with expertise in molecular biology techniques, DNA sequencing and bioinformatics analysis operates this lab in an integrative way. This platform was conceived not only to support the execution of the current projects of CeTICS but also to foster the development of new projects in Butantan Institute and in other research institutions.
Zelanis A, Andrade-Silva D, Rocha MM, Furtado MF, Serrano SM, Junqueira-de-Azevedo ILM, Ho PL. A Transcriptomic View of the Proteome Variability of Newborn and Adult Bothrops jararaca Snake Venoms. Plos Neglected Tropical Diseases. 2012;6(3):e1554.
Ching AT, Paes Leme AF, Zelanis A, Rocha MM, Furtado Mde F, Silva DA, Trugilho MR, da Rocha SL, Perales J, Ho PL, Serrano SM, Junqueira-de-Azevedo ILM. Venomics Profiling ofThamnodynastes strigatus Unveils Matrix Metalloproteinases and Other Novel Proteins Recruited to the Toxin Arsenal of Rear-Fanged Snakes. J Proteome Res. 2012 Feb 3;11(2):1152-62.
Leão LI, Ho PL, Junqueira-de-Azevedo ILM. Transcriptomic basis for an antiserum against Micrurus corallinus (coral snake) venom.BMC Genomics. 2009 Mar 16;10:112.
Junqueira-de-Azevedo ILM, Ching AT, Carvalho E, Faria F, Nishiyama MY Jr, Ho PL, Diniz MR. Lachesis muta (Viperidae) cDNAs reveal diverging pitviper molecules and scaffolds typical of cobra (Elapidae) venoms: implications in snake toxin repertoire evolution..Genetics (Austin), Estados Unidos. 2006 Jun;173(2):877-89.
Ching AT, Rocha MM, Paes Leme AF, Pimenta DC, de Fátima D Furtado M, Serrano SM, Ho PL, Junqueira-de-Azevedo ILM. Some aspects of the venom proteome of the Colubridae snake Philodryas olfersii revealed from a Duvernoy s (venom) gland transcriptome.FEBS Letters. 2006 Aug 7;580(18):4417-22.
Junqueira-de-Azevedo ILM, Ho PL. A survey of gene expression and diversity in the venom glands of the pitviper snake Bothrops insularis through the generation of Expressed Sequence Tags (ESTs).Gene (Amsterdam).2002 Oct 16;299(1-2):279-91.
Junqueira-de-Azevedo ILM, Farsky SHP, Oliveira MLS, Ho PL Molecular Cloning and Expression of a Functional Snake Venom Vascular Endothelium Growth Factor (VEGF) from the Bothrops insularis Pit Viper. A New Member of the VEGF Family of Proteins. The Journal of Biological Chemistry. 2001 Oct 26;276(43):39836-42.