Laboratory of Mass Spectrometry based Proteomics
Ismael Feitosa Lima
Regiane Spirandelli da Silva
Eduardo Shigueo Kitano, PhD Student
Ana Karina de Oliveira, PhD Student
Luciana Bertholim Nasciben, MSc Student
Edson Takeshi Yamashiro, MSc Student
Débora Andrade Silva, MSc Student
Dyoni Matias de Oliveira, MSc Student
Mariana Lopes de Camargo, Undergraduate Student
Daniel Ferreira Lopes, Undergraduate Student
Marcia Yuki Yoshioka, Undergraduate Student
The mass spectrometry-based proteomics lab has instrumentation and protocols for the development of research projects with established support from Instituto Butantan. The lab provides collaboration, consulting, and equipment to research investigators of Instituto Butantan and other public research institutions. In the last few years, lab investigators have been leading a strong training program for students and post docs aiming to progressively integrate projects using mass spectrometric-based proteomics towards a systemic view of biological phenomena. The amount and complexity of the data generated by the lab requires an active investigation and support staff to address the needs of the mass spectrometry-based research projects. The LETA-CeTICS bioinformatics group is actively engaged in both systems and software development to facilitate the analysis and correlation of mass spectral data and genomic/transcriptomic information, and also provides systems support and storage management to members of the laboratory. In this respect, a platform for interdisciplinary research that allows quantitative and qualitative-omics analyses and mathematical modeling of signaling networks (CeTICSdb) is currently under development at the CeTICS.
We expect to gain a competitive advantage in quantitative mass spectrometry-based proteomic analyses of cells and tissues that will enable us to understand complex signaling events triggered by venom proteinases and growth factors. Currently we are using protocols and tools to elucidate proteolytic mechanisms (degradomics) in platelets and components of the extracellular matrix, signaling effects of fibroblast growth factor 2 on the Y1 malignant cell line, especially on nuclei and chromatin, and for the characterization of new snake venom proteomes including their post-translational modifications.
Paes Leme AF, Kitano ES, Furtado MF, Valente RH, Camargo AC, Ho PL, Fox JW, Serrano SM. Analysis of the subproteomes of proteinases and heparin-binding toxins of eight Bothrops venoms. Proteomics.2009 Feb;9(3):733-45.
Zelanis A, Tashima AK, Rocha MM, Furtado MF, Camargo AC, Ho PL, Serrano SM. Analysis of the ontogenetic variation in the venom proteome/peptidome of Bothrops jararaca reveals different strategies to deal with prey. J. Proteome Res. 2010 May 7;9(5):2278-91.
Zelanis A, Tashima AK, Pinto AF, Paes Leme AF, Stuginski DR, Furtado MF, Sherman NE, Ho PL, Fox JW, Serrano SM. Bothrops jararaca venom proteome rearrangement upon neonate to adult transition. Proteomics.2011 Nov;11(21):4218-28.
Zelanis A, Serrano SM, Reinhold VN. N-glycome profiling of Bothrops jararaca newborn and adult venoms. J Proteomics.2012 Jan 4;75(3):774-82.
Paes Leme AF, Sherman NE, Smalley DM, Sizukusa LO, Oliveira AK, Menezes MC, Fox JW, Serrano SM. Hemorrhagic activity of HF3, a snake venom metalloproteinase: insights from the proteomic analysis of mouse skin and blood plasma. J Proteome Res.2012 Jan 1;11(1):279-91.
Tashima AK, Zelanis A, Kitano ES, Ianzer D, Melo RL, Rioli V, Sant'anna SS, Schenberg AC, Camargo AC, Serrano SM. Peptidomics of three Bothrops snake venoms: insights into the molecular diversification of proteomes and peptidomes. Mol Cell Proteomics.2012 Nov;11(11):1245-62.
Dias GS, Kitano ES, Pagotto AH, Sant'anna SS, Rocha MM, Zelanis A, Serrano SM. Individual variability in the venom proteome of juvenile Bothrops jararaca specimens. J Proteome Res.2013 Oct 4;12(10):4585-98.